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Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
21/12/2015 |
Data da última atualização: |
16/04/2018 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
TURCHETTO, C.; GONZÁLES, H. H. S.; TORRES, G. A. M.; NHANI, A.; CONSOLI, L.; BINNECK, E. |
Afiliação: |
ANTONIO NHANI JUNIOR, CNPT; LUCIANO CONSOLI, CNPT; ELISEU BINNECK, CNPSO. |
Título: |
Suppression subtractive hybridization analysis of genes regulated by magnaporthe oryzae infection in wheat adult plants. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE GENÉTICA, 61., 2015, Águas de Lindóia. Pós-genômica: [resumos]. |
Páginas: |
p. 34. |
Idioma: |
Inglês |
Conteúdo: |
Blast (also known as brusone), caused by the fungus Magnaporthe oryzae, is a serious disease of wheat (Triticum aestivum) across central and southern Brazil. The pathogen is a hemibiotrophic ascomycete that attacks several grass species. The disease was first described in rice in 1600 in China and it was reported infecting wheat ears in 1985 in Paraná state, Brazil, and since spread to all growing-regions in the country. Currently has been also reported on wheat fields in Bolivia, Paraguay and Argentina. The rice blast disease has emerged as a model for the study of phytopathogenic fungi showing that this pathogen initially colonizes host tissues as a biotroph, without causing detectable symptoms. Approximately 72?96 h after infection, lesions become apparent in the plant, characterizing the necrotrophic growth of M. oryzae. In wheat plants, depending on the developmental stage at which infection occurs blast can be devastating. Infected heads produce small and wrinkled grains with low specific weight. Few cultivars are described as resistant to wheat blast and fungicides have low control efficiency of the disease. Little is known about the molecular mechanism of wheat resistance to the pathogen. Here, we investigated the responses of wheat to M. oryzae infection in reproductive stage at 40 h after inoculation. The aim of this study was to identify genes that are differentially up- or downregulated in adults plants of Triticum aestivum infected with Magnaporthe oryzae. For this, we used a suppression subtractive hybridization (SSH) approach. A total of 420 high-quality contigs were isolated, 415 of them were mapped in Triticum aestivum genome. The 420 contigs were searched against the non-redundant nucleotide and protein databases in GenBank to predict the function for the corresponding genes. Fifty-five contigs corresponded to defense-related genes. We used the quantitative RT-PCR analysis to validate the differential expression patterns for 16 Triticum aestivum genes between control and inoculated spikes. Nine genes presented higher transcript levels under inoculation, including one gene previously described as responsive to Magnaporthe infection on wheat seedlings. This gene coding one protein membrane-associated that may increase the adhesion of the plasma membrane to the cell wall during pathogen infection. In contrast, the other 7 genes presented higher expression in mock-inoculated spikes. The study of these genes and the associated defense mechanisms can provide a significant advance in our understanding of the putative determinants of the resistance mechanisms of this wheat resistant genotype. MenosBlast (also known as brusone), caused by the fungus Magnaporthe oryzae, is a serious disease of wheat (Triticum aestivum) across central and southern Brazil. The pathogen is a hemibiotrophic ascomycete that attacks several grass species. The disease was first described in rice in 1600 in China and it was reported infecting wheat ears in 1985 in Paraná state, Brazil, and since spread to all growing-regions in the country. Currently has been also reported on wheat fields in Bolivia, Paraguay and Argentina. The rice blast disease has emerged as a model for the study of phytopathogenic fungi showing that this pathogen initially colonizes host tissues as a biotroph, without causing detectable symptoms. Approximately 72?96 h after infection, lesions become apparent in the plant, characterizing the necrotrophic growth of M. oryzae. In wheat plants, depending on the developmental stage at which infection occurs blast can be devastating. Infected heads produce small and wrinkled grains with low specific weight. Few cultivars are described as resistant to wheat blast and fungicides have low control efficiency of the disease. Little is known about the molecular mechanism of wheat resistance to the pathogen. Here, we investigated the responses of wheat to M. oryzae infection in reproductive stage at 40 h after inoculation. The aim of this study was to identify genes that are differentially up- or downregulated in adults plants of Triticum aestivum infected with Magnaporthe oryzae. For t... Mostrar Tudo |
Thesagro: |
Trigo. |
Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/135952/1/suppression.binneck.2015.pdf
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Marc: |
LEADER 03294nam a2200193 a 4500 001 2032054 005 2018-04-16 008 2015 bl uuuu u00u1 u #d 100 1 $aTURCHETTO, C. 245 $aSuppression subtractive hybridization analysis of genes regulated by magnaporthe oryzae infection in wheat adult plants.$h[electronic resource] 260 $aIn: CONGRESSO BRASILEIRO DE GENÉTICA, 61., 2015, Águas de Lindóia. Pós-genômica: [resumos].$c2015 300 $ap. 34. 520 $aBlast (also known as brusone), caused by the fungus Magnaporthe oryzae, is a serious disease of wheat (Triticum aestivum) across central and southern Brazil. The pathogen is a hemibiotrophic ascomycete that attacks several grass species. The disease was first described in rice in 1600 in China and it was reported infecting wheat ears in 1985 in Paraná state, Brazil, and since spread to all growing-regions in the country. Currently has been also reported on wheat fields in Bolivia, Paraguay and Argentina. The rice blast disease has emerged as a model for the study of phytopathogenic fungi showing that this pathogen initially colonizes host tissues as a biotroph, without causing detectable symptoms. Approximately 72?96 h after infection, lesions become apparent in the plant, characterizing the necrotrophic growth of M. oryzae. In wheat plants, depending on the developmental stage at which infection occurs blast can be devastating. Infected heads produce small and wrinkled grains with low specific weight. Few cultivars are described as resistant to wheat blast and fungicides have low control efficiency of the disease. Little is known about the molecular mechanism of wheat resistance to the pathogen. Here, we investigated the responses of wheat to M. oryzae infection in reproductive stage at 40 h after inoculation. The aim of this study was to identify genes that are differentially up- or downregulated in adults plants of Triticum aestivum infected with Magnaporthe oryzae. For this, we used a suppression subtractive hybridization (SSH) approach. A total of 420 high-quality contigs were isolated, 415 of them were mapped in Triticum aestivum genome. The 420 contigs were searched against the non-redundant nucleotide and protein databases in GenBank to predict the function for the corresponding genes. Fifty-five contigs corresponded to defense-related genes. We used the quantitative RT-PCR analysis to validate the differential expression patterns for 16 Triticum aestivum genes between control and inoculated spikes. Nine genes presented higher transcript levels under inoculation, including one gene previously described as responsive to Magnaporthe infection on wheat seedlings. This gene coding one protein membrane-associated that may increase the adhesion of the plasma membrane to the cell wall during pathogen infection. In contrast, the other 7 genes presented higher expression in mock-inoculated spikes. The study of these genes and the associated defense mechanisms can provide a significant advance in our understanding of the putative determinants of the resistance mechanisms of this wheat resistant genotype. 650 $aTrigo 700 1 $aGONZÁLES, H. H. S. 700 1 $aTORRES, G. A. M. 700 1 $aNHANI, A. 700 1 $aCONSOLI, L. 700 1 $aBINNECK, E.
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Embrapa Soja (CNPSO) |
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Registros recuperados : 51 | |
21. | | SILVA, V. R. da; ORTIGARA, C.; DEBIASI, H.; MORAES, M. T. de; LUZ, F. B.; FRANCHINI, J. C. Retenção e perda de água por evaporação de um Latossolo Vermelho sob diferentes níveis de compactação. In: REUNIÃO SUL-BRASILEIRA DE CIÊNCIA DO SOLO, 10., 2014, Pelotas. Fatos e mitos em ciência do solo. Pelotas: Sociedade Brasileira de Ciência do Solo; UFPEL, 2014. 1 pen drive.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
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22. | | MORAES, M. T. de; DEBIASI, H.; CARLESSO, R.; SILVA, V. R. da; FRANCHINI, J. C.; LEVIEN, R. Qualidade física de um Latossolo Vermelho avaliada por meio da curva de resistência do solo à penetração. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 34., 2013, Florianópolis. Ciência do solo: para quê e para quem? Anais... Florianópolis: SBCS, 2013. 4 p.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
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23. | | MORAES, M. T. de; SILVA, V. R. da; CHERUBIN, M. R.; CARLESSO, R.; DEBIASI, H.; LEVIEN, R. Charges in a rhodic hapludox under no-tillage and urban wast compost in the northwest of Rio Grande do Sul, Brazil. Revista Brasileira de Ciência do Solo, Viçosa, MG, v. 38, N. 4, p. 1327-1336, Jul./Aug. 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Soja. |
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24. | | DEBIASI, H.; MORAES, M. T. de; FRANCHINI, J. C.; DIAS, W. P.; SILVA, J. F. V.; RIBAS, L. N. Monitamento da fertilidade do solo e da ocorrência do nematoide das lesoes radiculares em soja no Mato Grosso. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 33., 2011, Uberlândia. Solos nos biomas brasileiros: sustentabilidade e mudanças climáticas: anais. Uberlândia: SBCS: UFU: ICIAG, 2011. 4 p. 1 CD-ROM. Título correto: Monitoramento da fertilidade do solo e da ocorrência do nematoide das lesoes radiculares em soja no Mato Grosso.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
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26. | | MORAES, M. T. de; DEBIASI, H.; FRANCHINI, J. C.; SILVA, V. R. da; CARLESSO, R.; LUZ, F. B. da. Curva de retenção de água de um latossolo sob estados de compactação. In: REUNIÃO BRASILEIRA DE MANEJO E CONSERVAÇÃO DO SOLO E DA ÁGUA, 19., 2012, Lages. Conservação do solo e da água no Brasil: preceitos e ações no ensino, na pesquisa e na extensão. Lages: SBCS: UDESC: IF: EPAGRI, 2012.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
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27. | | MORAES, M. T. de; DEBIASI, H.; FRANCHINI, J. C.; SILVA, V. R. da; CARLESSO, R.; LUZ, F. B. da. Curva de retenção de água de um Latossolo sob estados de compactação. In: REUNIÃO BRASILEIRA DE MANEJO E CONSERVAÇÃO DO SOLO E DA ÁGUA, 19., 2012, Lages. Conservação do solo e da água no Brasil: preceitos e ações no ensino, na pesquisa e na extensão: [anais]. [S.l.]: SBCS, 2012. 1 CD-ROM.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
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28. | | MORAES, M. T. de; DEBIASI, H.; CARLESSO, R.; SILVA, V. R. da; FRANCHINI, J. C.; LEVIEN, R. Estado de compactação de um Latossolo Vermelho com sistemas de manejo e modelos de produção de longo prazo. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 34., 2013, Florianópolis. Ciência do solo: para quê e para quem? Anais... Florianópolis: SBCS, 2013. 4 P.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
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29. | | MORAES, M. T. de; DEBIASI, H.; FRANCHINI, J. C.; BONETTI, J. de A.; LEVIEN, R.; SCHNEPF, A.; LEITNER, D. Mechanical and Hydric Stress Effects on Maize Root System Development at Different Soil Compaction Levels Frontiers in Plant Science, v. 10, artigo. 1358, out. 2019. 18 p.Tipo: Artigo em Periódico Indexado |
Biblioteca(s): Embrapa Soja. |
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30. | | MORAES, M. T. de; BENGOUGH, A. G.; DEBIASI, H.; FRANCHINI, J. C.; LEVIEN, R.; SCHNEPF, A.; LEITNER, D. Mechanistic framework to link root growth models with weather and soil physical properties, including example applications to soybean growth in Brazil. Plant and Soil, v. 42, n. 8, p. 67-92, 2018.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Soja. |
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31. | | LUZ, F. B. da; MORAES, M. T. de; SILVA, V. R. da; DEBIASI, H.; FRANCHINI, J. C.; ORTIGARA, C. Tempo de escarificação e adoção do sistema plantio direto e a relação com a densidade do solo máxima. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 34., 2013, Florianópolis. Ciência do solo: para quê e para quem? Anais... Florianópolis: SBCS, 2013. 4 p.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
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32. | | TREIN, C. R.; MORAES, M. T. de; LEVIEN, R.; MAZURANA, M.; ANGHIGNONI, I.; DEBIASI, H.; BONETTI, J. A. Tillage systems with controlled traffic and limits to maize production. In: CIOSTA, 36., CIGR SECTION, 5. 2015, Saint Petersburg. Environmentally friendly agriculture and forestry for future generations. Saint Peterburg: Saint-Petersburg State Agrarian University, 2015. p. 818-823.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
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33. | | ZULPO, L.; MORAES, M. T. de; DEBIASI, H.; FRANCHINI, J. C.; LUZ, F. B. da; LEVIEN, R. Sistemas de manejo do solo e de culturas em latossolo vermelho e sua relação com a produtividade de soja. In: REUNIÃO SUL-BRASILEIRA DE CIÊNCIA DO SOLO, 10., 2014, Pelotas. Fatos e mitos em ciência do solo. Pelotas: Sociedade Brasileira de Ciência do Solo; UFPEL, 2014. 1 pen drive.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
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34. | | MORAES, M. T. de; DEBIASI, H.; FRANCHINI, J. C.; MASTROBERTI, A. A.; LEVIEN, R.; LEITNER, D.; SCHNEPF, A. Soil compaction impacts soybean root growth in an Oxisol from subtropical Brazil Soil & Tillage Research, v. 200, 104611, 2020.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Soja. |
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37. | | BALBINOT JUNIOR, A. A.; DEBIASI, H.; FRANCHINI, J. C.; OLIVEIRA, M. A. de; COELHO, A. E.; MORAES, M. T. de. Soybean yield, seed protein and oil concentration, and soil fertility affected by off-season crops. European Journal of Agronomy, v. 153, 127039, 2024. 11 p.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Soja. |
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38. | | ARNUTI, F.; SILVA, V. R. da; MORAES, M. T. de; PIOVESAN, M. T.; MIGLIORINI, P.; CADORIN, A. M. R. Utilização de lodo na cultura do girassol (Helianthus annuuus L.) In: REUNIÃO NACIONAL DE PESQUISA DO GIRASSOL, 18., SIMPÓSIO NACIONAL SOBRE A CULTURA DO GIRASSOL, 6., 2009. Pelotas. Anais... Pelotas: Embrapa Clima Temperado, 2009. p. 274-281. Editado por Ana Claudia Barneche de Oliveira, Ana Paula Afonso Scheneid da Rosa e Márcia Vizzotto.Biblioteca(s): Embrapa Clima Temperado. |
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40. | | FRANCHINI, J. C.; MORAES, M. T. de; DEBIASI, H.; DIAS, W. P.; RIBAS, L. N.; SILVA, J. F. V. Variabilidade espacial de atributos químicos do solo e relação com os danos pelo nematoide das lesões radiculares em soja. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 33., 2011, Uberlândia. Solos nos biomas brasileiros: sustentabilidade e mudanças climáticas: anais. Uberlândia: SBCS: UFU: ICIAG, 2011. 4 p. 1 CD-ROM.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
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Registros recuperados : 51 | |
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